LSBio
IHC-plus™ DNASE1 / DNase I Antibody

Anti-DNase I antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ DNASE1 / DNase I Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all DNASE1 / DNase I antibodies →
$470.00each
Catalog Number: LS-B4846-50

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Overview

Description
DNase I antibody LS-B4846 is an unconjugated rabbit polyclonal antibody to human DNase I (DNASE1). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Application
ICC, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Deoxyribonuclease I | Deoxyribonuclease i precursor | DNase I | DNL1 | DRNI | Deoxyribonuclease-1 | DNase I, lysosomal | DNASE1
Recommended Dilution: IF/ICC
1:100 - 1:1000
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:500 - 1:3000
UniProt Number
P24855
SwissProt
P24855

Target

Target
DNASE1 / DNase I
Antigen Species
Hu
Specificity
Human DNase I. Predicted cross-reactivity based on amino acid sequence homology: rabbit (81%), dogs (81%).
Epitope
Human DNase I. Predicted cross-reactivity based on amino acid sequence homology: rabbit (81%), dogs (81%).
Gene Name
DNASE1

Antibody

Host Species
Rb
Clonality
Polyclonal
Origin Species
Hu

Immunogen

Immunogen
Recombinant protein corresponding to human DNase I.

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
1X PBS, pH 7, 0.025% ProClin™ 300, 20% Glycerol
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer
1X PBS
TNFα Amplifies DNaseI Expression in Renal Tubular Cells while IL-1β Promotes Nuclear DNaseI Translocation in an Endonuclease-Inactive Form. Thiyagarajan D, Rekvig OP, Seredkina N. PloS one. 2015;10:e0129485. PubMed: 26065428 PMC: PMC4465975

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