DNASE1 / DNase I Antibody
Catalog Number: LS-C663654
6 sizes / from $312.00
Antibody: Mouse Monoclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Anti-DNase I antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC-plus™ DNASE1 / DNase I Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$470.00each
Catalog Number: LS-B4846-50
Catalog Number: LS-B4846
For bulk orders or custom formulations:
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- Description
- DNase I antibody LS-B4846 is an unconjugated rabbit polyclonal antibody to human DNase I (DNASE1). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
- Application
- ICC, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Deoxyribonuclease I | Deoxyribonuclease i precursor | DNase I | DNL1 | DRNI | Deoxyribonuclease-1 | DNase I, lysosomal | DNASE1
- Recommended Dilution: IF/ICC
- 1:100 - 1:1000
- Recommended Dilution: IHC-P
- 10 µg/ml
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- P24855
- NCBI GenBank
- NM_005223 | NP_005214.2
- SwissProt
- P24855
Target
- Target
- DNASE1 / DNase I
- Antigen Species
- Human
- Specificity
- Human DNase I. Predicted cross-reactivity based on amino acid sequence homology: rabbit (81%), dogs (81%).
- Epitope
- Human DNase I. Predicted cross-reactivity based on amino acid sequence homology: rabbit (81%), dogs (81%).
- Gene Name
- DNASE1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant protein corresponding to human DNase I.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 1X PBS, pH 7, 0.025% ProClin™ 300, 20% Glycerol
- Volume
- 50 Microliter
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 1X PBS
TNFα Amplifies DNaseI Expression in Renal Tubular Cells while IL-1β Promotes Nuclear DNaseI Translocation in an Endonuclease-Inactive Form. Thiyagarajan D, Rekvig OP, Seredkina N. PloS one. 2015;10:e0129485.
PubMed: 26065428 · PMC: PMC4465975
IL-1ß Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene. Thiyagarajan D, Pedersen HL, Seredkina N, Horvei KD, Arranz L, Sonneveld R, Nijenhuis T, van der Vlag J, Rekvig OP. Frontiers in cell and developmental biology. 2018 February;6:7.
PubMed: 29468159 · PMC: PMC5807897
Lupus nephritis: low urinary DNase I levels reflect loss of renal DNase I and may be utilized as a biomarker of disease progression. Pedersen HL, Horvei KD, Thiyagarajan D, Norby GE, Seredkina N, Moroni G, Eilertsen GØ, Holdaas H, Strøm EH, Bakland G, Meroni PL, Rekvig OP. The journal of pathology Clinical research. 2018 July;4:193-203.
PubMed: 29624903 · PMC: PMC6065113
