Immunofluorescence staining of DNASE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DNASE1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
DNASE1 / DNase I Antibody (clone 006, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general) |
| Reactivity: | Human |
| Format: | Liquid |
$164.00each
Catalog Number: LS-C818939-20
Catalog Number: LS-C818939
For bulk orders or custom formulations:
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- Description
- DNase I antibody LS-C818939 is an unconjugated rabbit monoclonal antibody to human DNase I (DNASE1). Validated for ELISA, ICC and IF.
- Application
- ELISA, ICC, IF, IHC
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Deoxyribonuclease I | Deoxyribonuclease i precursor | DNase I | DNL1 | DRNI | Deoxyribonuclease-1 | DNase I, lysosomal | DNASE1
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:20 - 1:100
- UniProt Number
- P24855
- NCBI GenBank
- NM_005223 | NP_005214.2
- SwissProt
- P24855
Target
- Target
- Human DNASE1 / DNase I
- Antigen Species
- Human
- Epitope
- Human DNase I
- Gene Name
- DNASE1
Antibody
- Host Species
- Rabbit
- Clonality
- Monoclonal
- Clone
- 006
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human DNase1 / Deoxyribonuclease I / DNL1 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 20 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS