LSBio
DLL4 Antibody (Internal)

Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

DLL4 Antibody (Internal)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human DLL4 antibodies →
$579.00each
Catalog Number: LS-C19035-100

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Overview

Description
DLL4 antibody LS-C19035 is an unconjugated rabbit polyclonal antibody to human DLL4 (Internal). Validated for ELISA, IHC and WB. Cited in 2 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Mouse, Non-Human Primate, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Delta-like 4 protein | Delta like 4 | Delta-like 4 (Drosophila) | Delta-like 4 homolog | DLL4 | Drosophila Delta homolog 4 | Notch ligand DLL4 | Delta 4 | Delta ligand 4 | Delta-like protein 4 | Delta4 | Hdelta2 | Notch ligand delta-2
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: WB
1:500 - 1:2500
UniProt Number
Q9NR61
SwissProt
Q9NR61

Target

Target
Human DLL4
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse, rat and chimpanzee DELTA-4 based on a 91% homology to the core 12 amino acid sequence within the 14 amino acid immunogen sequence. Cross reactivity with DELTA-4 homologues from other sources has not been determined.
Gene Name
DLL4

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
Cholera toxin disrupts barrier function by inhibiting exocyst-mediated trafficking of host proteins to intestinal cell junctions. Guichard A, Cruz-Moreno B, Cruz-Moreno BC, Aguilar B, van Sorge NM, Kuang J, Kurkciyan AA, Wang Z, Hang S, Pineton de Chambrun GP, McCole DF, Watnick P, Nizet V, Bier E. Cell host & microbe. 2013 Sep;14:294-305. PubMed: 24034615 PMC: PMC3786442

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