Fluorescent image of HeLa cell stained with DDIT3 Antibody (C-term A135). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). DDIT3 immunoreactivity is localized to Cytoplasm significantly.
DDIT3 / CHOP Antibody (aa120-149)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C167320-400
Catalog Number: LS-C167320
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- Description
- CHOP antibody LS-C167320 is an unconjugated rabbit polyclonal antibody to CHOP (DDIT3) (aa120-149) from human. It is reactive with human and mouse. Validated for Flow, IF and WB.
- Application
- FC, IF, IHC, WB
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Bovine, Hamster
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CHOP10 | DDIT-3 | CHOP | GADD153 | C/EBP zeta | C/EBP-homologous protein | C/EBP-homologous protein 10 | CHOP-10 | DDIT3
- Recommended Dilution: FC
- 1:10 - 1:50
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P35638
- NCBI GenBank
- NM_004083 | NP_004074.2
- SwissProt
- P35638
Target
- Target
- Human DDIT3 / CHOP
- Antigen Species
- Human
- Epitope
- This DDIT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-149 amino acids from the C-terminal region of human DDIT3.
- Gene Name
- DDIT3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS