IHC analysis of CTNNB1 using anti-CTNNB1 antibody. CTNNB1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTNNB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CTNNB1 / Beta Catenin Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C661899-100
Catalog Number: LS-C661899
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- Description
- Beta Catenin antibody LS-C661899 is an unconjugated mouse monoclonal antibody to human Beta Catenin (CTNNB1). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Beta-catenin | Catenin beta-1 | CTNNB | CTNNB1 | Beta Catenin
- UniProt Number
- P35222
- NCBI GenBank
- NM_001904 | NP_001895.1
- SwissProt
- P35222
Target
- Target
- Human CTNNB1 / Beta Catenin
- Antigen Species
- Human
- Epitope
- Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level).
- Gene Name
- CTNNB1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
Immunogen
- Immunogen
- E. coli-derived human beta Catenin recombinant protein (Position: A2-K233).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- NaCl