Western Blot of rabbit Anti-Carboxypeptidase B antibody. Lane 1: Carboxypeptidase B. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carboxypeptidase B primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 35 kDa, 35 kDa for Carboxypeptidase B. Other band(s): Carboxypeptidase B splice variants and isoforms.
CPB / Carboxypeptidase B Antibody (Liquid)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Pig |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744952-25
Catalog Number: LS-C744952
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- Description
- Carboxypeptidase B antibody LS-C744952 is an unconjugated rabbit polyclonal antibody to pig Carboxypeptidase B (CPB). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Por
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Carboxypeptidase B | Carboxypeptidase B1 (tissue) | CPB | CPB1 | Pancreatic carboxypeptidase B | PASP | Procarboxypeptidase B | Protaminase | Pancreas-specific protein | PCPB | Tissue carboxypeptidase B
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IHC
- 1:250 - 1:1000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P15086
- NCBI GenBank
- NM_001871 | NP_001862.2
- SwissProt
- P15086
Target
- Target
- Pig CPB / Carboxypeptidase B
- Antigen Species
- Pig
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas]. Cross reactivity against Carboxypeptidase B from other tissues and species may occur but have not been specifically determined.
- Gene Name
- CPB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Carboxypeptidase B [Porcine Pancreas].
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl