Western Blot of rabbit anti-Carboxypeptidase B Antibody Peroxidase Conjugated. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carboxypeptidase B Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 47 kDa, observed at 35 kDa for Carboxypeptidase B. Expected molecular weight for processed protein is ~34.7 kDa.
CPB / Carboxypeptidase B Antibody (Liquid, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Pig |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745021-25
Catalog Number: LS-C745021
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- Description
- Carboxypeptidase B antibody LS-C745021 is an HRP-conjugated rabbit polyclonal antibody to pig Carboxypeptidase B (CPB). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Por
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Carboxypeptidase B | Carboxypeptidase B1 (tissue) | CPB | CPB1 | Pancreatic carboxypeptidase B | PASP | Procarboxypeptidase B | Protaminase | Pancreas-specific protein | PCPB | Tissue carboxypeptidase B
- Recommended Dilution: ELISA
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:250 - 1:1000
- UniProt Number
- P15086
- NCBI GenBank
- NM_001871 | NP_001862.2
- SwissProt
- P15086
Target
- Target
- Pig CPB / Carboxypeptidase B
- Antigen Species
- Pig
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas]. Cross reactivity against Carboxypeptidase B from other tissues and species is unknown.
- Gene Name
- CPB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Carboxypeptidase B [Porcine Pancreas].
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl