LSBio
Chicken Anti-Human IgG Secondary Antibody (AP)

Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated Western Blot. Western Blot of Chicken anti-Human IgG Antibody Alkaline Phosphatase Conjugated Lane 1: Human IgG Load: 100 ng per lane Secondary antibody: Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kD, 55 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Chicken Anti-Human IgG Secondary Antibody (AP)

Secondary AntibodyLSBio Guarantee
Antibody:Chicken Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
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$398.00each
Catalog Number: LS-C60659-1

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Overview

Description
Human IgG antibody LS-C60659 is an AP-conjugated chicken polyclonal antibody. Validated for ELISA, IHC and WB.
Application
DB, ELISA, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:500 - 1:2500

Target

Target
Human IgG
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Chicken Serum, Human IgG and Human Serum.

Antibody

Host Species
Chicken
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Human IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Alkaline Phosphatase
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.