Detection of human and mouse FLIP in 293T cells transfected with a human (lane 2) or mouse FLIPL (lane 3) expression plasmid using anti-FLIP, mAb (Dave-2). Untransfected cells (lane 1). Top arrows indicate full length FLIP, lower arrows indicate cleaved FLIP products. . Method: Cell extracts from cells (5x104) transfected with human or mouse FLIP expression plasmid were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-FLIP, mAb (Dave-2) at 1microg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
CFLAR / FLIP Antibody (aa1-480, clone Dave-2)
Primary AntibodyLSBio Guarantee
| Antibody: | Rat Monoclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$497.00each
Catalog Number: LS-C344933-100
Catalog Number: LS-C344933
For bulk orders or custom formulations:
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- Description
- FLIP antibody LS-C344933 is an unconjugated rat monoclonal antibody to FLIP (CFLAR) (aa1-480) from human. It is reactive with human and mouse. Validated for IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- C-FLIPR | C-FLIPS | C-FLIP | CASP8AP1 | Cflip | CLARP | CASH | Caspase homolog | Caspase-eight-related protein | CFLAR | Flice inhibitory protein | I-FLICE | Inhibitor of FLICE | FLAME | FLAME1 | MRIT | Usurpin beta | C-FLIPL | Casper | FLAME-1 | FLIP | Usurpin
- UniProt Number
- O15519
- NCBI GenBank
- NM_003879 | NP_003870.4
- SwissProt
- O15519
Target
- Target
- Human CFLAR / FLIP
- Antigen Species
- Human
- Epitope
- Recognizes an epitope (aa 1-200) present in both short (FLIP
- Gene Name
- CFLAR
Antibody
- Host Species
- Rat
- Clonality
- Monoclonal
- Clone
- Dave-2
- Isotype
- IgG2a
Immunogen
- Immunogen
- Recombinant human FLIP (aa 1-480).
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.02% Sodium Azide, 10% Glycerol
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS