IHC of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using Polyclonal Antibody to FLIP (long and short) at 1:2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-Eosin counterstain.
CFLAR / FLIP Antibody (aa75-92)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human, Non-Human Primate |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C148270-50
Catalog Number: LS-C148270
For bulk orders or custom formulations:
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- Description
- FLIP antibody LS-C148270 is an unconjugated rabbit polyclonal antibody to FLIP (CFLAR) (aa75-92) from human. It is reactive with human and monkey. Validated for IHC, IP and WB.
- Application
- IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-FLIPR | C-FLIPS | C-FLIP | CASP8AP1 | Cflip | CLARP | CASH | Caspase homolog | Caspase-eight-related protein | CFLAR | Flice inhibitory protein | I-FLICE | Inhibitor of FLICE | FLAME | FLAME1 | MRIT | Usurpin beta | C-FLIPL | Casper | FLAME-1 | FLIP | Usurpin
- Recommended Dilution: IHC-P
- 1:1000 - 1:5000
- Recommended Dilution: IP
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- O15519
- NCBI GenBank
- NM_003879 | NP_003870.4
- SwissProt
- O15519
Target
- Target
- Human CFLAR / FLIP
- Antigen Species
- Human
- Epitope
- The gene encoding FLIP produces, by alternative splicing, 24 different transcripts, all with introns, putatively encoding 22 different protein isoforms. The molecular weights of these isoforms may vary. Therefore, the molecular weight of FLIP observed by western blot may vary according to the isoforms present in the sample.
- Gene Name
- CFLAR
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A synthetic peptide corresponding to amino acids 75-92 (DRKAVETHLLRNPHLVS) of human FLIP, GenBank no. gi|12643547. This amino acid sequence is 100% conserved in FLIP long and short, and various FLIP isoforms as denoted in the NCBI BLAST data base. http://www. ncbi. nlm. nih. gov/blast /. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%); Gibbon (94%); Marmoset (88%); Panda (82%); Mouse (76%); Rat (71%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.05% sodium azide
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.