Anti-CDK9 pT29 Antibody - Western Blot. Western blot of affinity purified anti-CDK9 pT29 antibody shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution. Personal Communication, J. Brady, NCI, Bethesda, MD.

CDK9 Antibody (phospho-Thr29)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Liquid

$579.00each

Catalog Number: LS-C60108-100

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Overview

Description: CDK9 antibody LS-C60108 is an unconjugated rabbit polyclonal antibody to CDK9 (pThr29) from human. It is reactive with human, mouse and rat. Validated for ELISA, IP and WB.
Application: ELISA, IP, WB
Reactivity: Hu
Predicted Reactivity: Mouse, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: C-2k | CDC2L4 | CDC2-related kinase | Cell division protein kinase 9 | Cyclin-dependent kinase 9 | CDC2-related protein kinase | PITALRE | TAK | CDK9 | CTK1
Recommended Dilution: ELISA: 1:5000 - 1:24000
Recommended Dilution: IP: 1:100
Recommended Dilution: WB: 1:200 - 1:2000
UniProt Number: P50750
NCBI GenBank: NM_001261 | NP_001252.1
SwissProt: P50750

Target

Target: Human CDK9
Antigen Species: Human
Epitope: This antibody is specific for human CDK9 protein phosphorylated at T29. A BLAST analysis was used to suggest cross-reactivity with CDK9 from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with CDK9 from other sources has not been determined.
Gene Name: CDK9

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T29 in the human CDK9 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1.3 mg/mL
Preservative: Yes
Purification Method: Affinity Purified