LSBio
IHC-plus™ CDK9 Antibody (N-Terminus and C-Terminus)

Anti-CDK9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

IHC-plus™ CDK9 Antibody (N-Terminus and C-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$526.00each
Catalog Number: LS-B354-50

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Overview

Description
CDK9 antibody LS-B354 is an unconjugated rabbit polyclonal antibody to CDK9 (N-Terminus and C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 5 publications.
Application
ELISA, ICC, IHC, IHC-P, IP, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
C-2k | CDC2L4 | CDC2-related kinase | Cell division protein kinase 9 | Cyclin-dependent kinase 9 | CDC2-related protein kinase | PITALRE | TAK | CDK9 | CTK1
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC-P
1:500
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:3000
UniProt Number
P50750
SwissProt
P50750

Target

Target
CDK9
Antigen Species
Human
Specificity
Antiserum will specifically react with a 35 kDa cdk5 (p31) protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk5 (p31) from other species may also occur.
Epitope
Antiserum will specifically react with a 35 kDa cdk5 (p31) protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk5 (p31) from other species may also occur.
Gene Name
CDK9

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27Kip1, to regions involved in human cancer. Graa X, De Luca A, Sang N, Fu Y, Claudio PP, Rosenblatt J, Morgan DO, Giordano A. Cancer research. 1995;55:1199-205.

PubMed: 7882308


PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro. Graa X, De Luca A, Sang N, Fu Y, Claudio PP, Rosenblatt J, Morgan DO, Giordano A. Proceedings of the National Academy of Sciences of the United States of America. 1994;91:3834-8.

PubMed: 8170997 · PMC: PMC43676


Cloning of murine CDK9/PITALRE and its tissue-specific expression in development. Bagella L, MacLachlan TK, Buono RJ, Pisano MM, Giordano A, De Luca A. Journal of cellular physiology. 1998;177:206-13.

PubMed: 9766517


Binding of CDK9 to TRAF2. MacLachlan TK, Sang N, De Luca A, Puri PL, Levrero M, Giordano A. Journal of cellular biochemistry. 1998;71:467-78.

PubMed: 9827693


CDK9 (PITALRE): a multifunctional cdc2-related kinase. de Falco G, Giordano A. Journal of cellular physiology. 1998;177:501-6.

PubMed: 10092203

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