IHC analysis of Cdk6 using anti-Cdk6 antibody. Cdk6 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cdk6 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CDK6 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Lyophilized

$480.00each

Catalog Number: LS-C756393-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: CDK6 antibody LS-C756393 is an unconjugated rabbit polyclonal antibody to CDK6 from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Application: ELISA, IHC-P, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CDKN6 | Cell division protein kinase 6 | CDK6 | Cyclin-dependent kinase 6 | Crk2 | PLSTIRE
Recommended Dilution: ELISA: 0.1 - 0.5 ug/ml
Recommended Dilution: IHC-P: 0.5 - 1 µg/ml
Recommended Dilution: WB: 0.1 - 0.5 µg/ml
UniProt Number: Q00534
NCBI GenBank: NM_001259 | NP_001250.1
SwissProt: Q00534

Target

Target: Human CDK6
Antigen Species: Human
Epitope: No cross reactivity with other proteins.
Gene Name: CDK6

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: E. coli-derived human Cdk6 recombinant protein (Position: M1-A326).
Immunogen Type: Recombinant Immunogen

Format

Conjugate/Label: Unconjugated
Format: Lyophilized
Formulation: Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Recommended Storage Buffer: NaCl