Western blot analysis of extracts of various cell lines, using CDK6 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

CDK6 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$281.00each

Catalog Number: LS-C331529-20

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Overview

Description: CDK6 antibody LS-C331529 is an unconjugated rabbit polyclonal antibody to CDK6 from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP and WB.
Application: IF, IHC, IP, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CDKN6 | Cell division protein kinase 6 | CDK6 | Cyclin-dependent kinase 6 | Crk2 | PLSTIRE
Recommended Dilution: IF/ICC: 1:50 - 1:200
Recommended Dilution: IHC: 1:50 - 1:200
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q00534
NCBI GenBank: NM_001259 | NP_001250.1
SwissProt: Q00534

Target

Target: Human CDK6
Antigen Species: Human
Epitope: Human Cdk6
Gene Name: CDK6

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human CDK6 (NP_001250.1). MEKDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)