Immunofluorescence staining of CD58 in Raji cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD58 polyclonal antibody (dilution ratio 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.

CD58 Antibody (Preservative Free)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Western Blot
Reactivity:	Human
Format:	Liquid

$309.00each

Catalog Number: LS-C818136-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: CD58 antibody LS-C818136 is an unconjugated rabbit polyclonal antibody to human CD58. Validated for ELISA, ICC, IF and WB.
Application: ELISA, ICC, IF, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CD58 antigen | CD58 | AG3 | LFA-3 | LFA3 | Surface glycoprotein LFA-3 | Surface glycoprotein lfa3 | CD58 molecule
Recommended Dilution: ELISA: 1:25000 - 1:50000
Recommended Dilution: IF/ICC: 1:1500 - 1:50000
Recommended Dilution: WB: 1:500 - 1:1000
UniProt Number: P19256
NCBI GenBank: NM_001779 | NP_001770.1
SwissProt: P19256

Target

Target: Human CD58
Antigen Species: Human
Epitope: Human CD58
Gene Name: CD58

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant Human LFA-3 / CD58 Protein
Immunogen Type: Recombinant Immunogen

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.2 µm filtered solution in PBS
Volume: 100 Microliter
Preservative: No
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS