CD58 Antibody (Cytoplasmic Domain, clone BRIC5)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Flow Cytometry, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$392.00each

Catalog Number: LS-C45438-0.2

Unit Size

Quantity

For bulk orders or custom formulations:

Description: CD58 antibody LS-C45438 is an unconjugated mouse monoclonal antibody to human CD58 (Cytoplasmic Domain). Validated for Flow and WB.
Application: FC, IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: CD58 antigen | CD58 | AG3 | LFA-3 | LFA3 | Surface glycoprotein LFA-3 | Surface glycoprotein lfa3 | CD58 molecule
Recommended Dilution: FC: 1:10
UniProt Number: P19256
NCBI GenBank: NM_001779 | NP_001770.1
SwissProt: P19256

Target: Human CD58
Antigen Species: Human
Epitope: CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes[1]. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells[2]. There are approximately 5000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on hemopoietic cells in individuals with paroxysmal nocturnal hemoglobinuria. BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. Reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect hemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.
Gene Name: CD58

Storage Conditions: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.