Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
CD3D was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HuT78 Whole Cell Lysate. 2 uL anti-CD3D mouse monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD3D mouse monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa.
CD3D Antibody (clone 6B8D1G5, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$148.00each
Catalog Number: LS-C819744-20
Catalog Number: LS-C819744
For bulk orders or custom formulations:
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- Description
- CD3D antibody LS-C819744 is an unconjugated mouse monoclonal antibody to human CD3D. Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD3 delta | CD3d antigen | T-cell receptor T3 delta chain | T3D | CD3 antigen, delta subunit | CD3-DELTA | CD3D | OKT3, delta chain
- Recommended Dilution: ELISA
- 1:1000 - 1:2000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P04234
- NCBI GenBank
- NM_000732 | NP_000723.1
- SwissProt
- P04234
Target
- Target
- Human CD3D
- Antigen Species
- Human
- Epitope
- Human CD3D
- Gene Name
- CD3D
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 6B8D1G5
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human CD3D protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 20 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS
