Western blot analysis of extracts of various cell lines, using FCGR2A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

CD32A Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C331441-20

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Overview

Description: CD32A antibody LS-C331441 is an unconjugated rabbit polyclonal antibody to CD32A from human. It is reactive with human and mouse. Validated for IF, IHC and WB.
Application: IF, IHC, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CD32 | CD32 antigen | CDw32 | FCG2 | FcGR | FCGR2A1 | FcRII-a | Fc-gamma RII-a | Fc-gamma-RIIa | FcRII-c | IgG Fc receptor II-a | Igg fc receptor ii-c | Fc-gamma rii-c | FCGR2 | FCGR2A | Igg fc receptors ii-c | CD32A | IGFR2
Recommended Dilution: IF/ICC: 1:50 - 1:200
Recommended Dilution: IHC: 1:50 - 1:200
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P12318
NCBI GenBank: NM_021642 | NP_067674.2
SwissProt: P12318

Target

Target: Human CD32A
Antigen Species: Human
Epitope: Raised against amino acids 34-316 of human CD32A. This antibody has not been tested for cross reactivity with CD32B and may cross react.
Gene Name: FCGR2A

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-316 of human FCGR2A (NP_067674.2). QAAPPKAVLKLEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPMGIIVAVVIATAVAAIVAAVVALIYCRKKRISANSTDPVKAAQFEPPGRQMIAIRKRQLEETNNDYETADGGYMTLNPRAPTDDDKNIYLTLPPNDHVNSNN
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)