Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
CD32A Antibody (clone OTI9G5)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C114747-100
Catalog Number: LS-C114747
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- CD32A antibody LS-C114747 is an unconjugated mouse monoclonal antibody to human CD32A. Validated for IF, IHC, IP and WB.
- Application
- IF, IHC, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD32 | CD32 antigen | CDw32 | FCG2 | FcGR | FCGR2A1 | FcRII-a | Fc-gamma RII-a | Fc-gamma-RIIa | FcRII-c | IgG Fc receptor II-a | Igg fc receptor ii-c | Fc-gamma rii-c | FCGR2 | FCGR2A | Igg fc receptors ii-c | CD32A | IGFR2
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC-P
- 1:50
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- P12318
- NCBI GenBank
- NM_021642 | NP_067674.2
- SwissProt
- P12318
Target
- Target
- Human CD32A
- Antigen Species
- Human
- Epitope
- Reacts with human CD32A, not yet tested for reactivity with CD32B.
- Gene Name
- FCGR2A
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI9G5
- Isotype
- IgG1
Immunogen
- Immunogen
- Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 0.66 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
