Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY111 mCD3zeta (clone EM-55). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 (thus only pY111 remains native) 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 8: mut. mCD3zeta Y/F1-5
CD247 / CD3 Zeta Antibody (phospho-Tyr111, clone EM-55)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C662910-0.1
Catalog Number: LS-C662910
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- CD3 Zeta antibody LS-C662910 is an unconjugated mouse monoclonal antibody to CD3 Zeta (CD247) (pTyr111) from human. It is reactive with human and mouse. Validated for Flow and WB.
- Application
- FC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD3 Zeta | CD3H | CD3Z | CD247 antigen, zeta subunit | CD247 molecule | CD3zeta chain | T3Z | TCR zeta chain | TCRZ | T-cell receptor T3 zeta chain | CD247 | CD247 antigen | CD3-ZETA | CD3Q
- UniProt Number
- P20963
- NCBI GenBank
- NM_000734 | NP_000725.1
- SwissProt
- P20963
Target
- Target
- Human CD247 / CD3 Zeta
- Antigen Species
- Human
- Epitope
- The mouse monoclonal antibody EM-55 recognizes phosphorylated tyrosine 111 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
- Gene Name
- CD247
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- EM-55
- Isotype
- IgG1
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 15 mM Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C. Do not freeze.
- Recommended Storage Buffer
- PBS