Western blot analysis of lysates from Jurkat cells treated with UV 15', using CD3 zeta (Phospho-Tyr142) Antibody. The lane on the right is blocked with the phospho peptide.
CD247 / CD3 Zeta Antibody (phospho-Tyr142)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$341.00each
Catalog Number: LS-C198760-50
Catalog Number: LS-C198760
For bulk orders or custom formulations:
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- Description
- CD3 Zeta antibody LS-C198760 is an unconjugated rabbit polyclonal antibody to CD3 Zeta (CD247) (pTyr142) from human. It is reactive with human, mouse and rat. Validated for Peptide-ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD3 Zeta | CD3H | CD3Z | CD247 antigen, zeta subunit | CD247 molecule | CD3zeta chain | T3Z | TCR zeta chain | TCRZ | T-cell receptor T3 zeta chain | CD247 | CD247 antigen | CD3-ZETA | CD3Q
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P20963
- NCBI GenBank
- NM_000734 | NP_000725.1
- SwissProt
- P20963
Target
- Target
- Human CD247 / CD3 Zeta
- Antigen Species
- Human
- Epitope
- CD3 zeta (Phospho-Tyr142) Antibody detects endogenous levels of CD3 zeta only when phosphorylated at tyrosine142.
- Gene Name
- CD247
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic peptide derived from human CD3 zeta around the phosphorylation site of Tyr142. (AA Range: 111-160)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS|150 mM NaCl