IHC analysis of CCL8 using anti-CCL8 antibody. CCL8 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CCL8 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CCL8 / MCP2 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin |
| Reactivity: | Mouse |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C756573-100
Catalog Number: LS-C756573
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- Description
- MCP2 antibody LS-C756573 is an unconjugated rabbit polyclonal antibody to mouse MCP2 (CCL8). Validated for ELISA and IHC.
- Application
- ELISA, IHC-P
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-C motif chemokine 8 | CCL8 | HC14 | MCP2 | Monocyte chemotactic protein 2 | Scya10 | SCYA8 | Small-inducible cytokine A8 | MCP-2 | Chemokine (C-C motif) ligand 8
- Recommended Dilution: ELISA
- 1 - 5 µg/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- UniProt Number
- P80075
- NCBI GenBank
- NM_005623 | NP_005614.2
- SwissProt
- P80075
Target
- Target
- Mouse CCL8 / MCP2
- Antigen Species
- Mouse
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- CCL8
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived mouse CCL8 recombinant protein (Position: G24-P97).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
- Recommended Storage Buffer
- NaCl