Western blot using the protein-A purified Anti-MIP-1 (CCL4) antibody shows detection of recombinant swine MIP-1 (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with at 1:20,000 for 45 min at room temperature.
CCL4 / MIP-1 Beta Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Pig |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745273-25
Catalog Number: LS-C745273
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- Description
- MIP-1 Beta antibody LS-C745273 is an unconjugated rabbit polyclonal antibody to pig MIP-1 Beta (CCL4). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Por
- Predicted Reactivity
- Horse, Mammal (Other)
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ACT2 | Act-2 | AT744.1 | C-C motif chemokine 4 | Chemokine (C-C motif) ligand 4 | CC chemokine ligand 4 | HC21 | LAG1 | MIP1B | MIP1B1 | LAG-1 | SCYA2 | Secreted protein G-26 | SIS-gamma | MIP-1-beta | MIP-1-beta(1-69) | Small-inducible cytokine A4 | T-cell activation protein 2 | CCL4 | G-26 | Lymphocyte-activation gene 1 | PAT 744 | Protein H400 | SCYA4
- Recommended Dilution: ELISA
- 1:5000
- Recommended Dilution: WB
- 1:500
- UniProt Number
- P13236
- NCBI GenBank
- NM_002984 | NP_002975.1
- SwissProt
- P13236
Target
- Target
- Pig CCL4 / MIP-1 Beta
- Antigen Species
- Pig
- Epitope
- This antibody is specific for swine CCL4 protein. A BLAST analysis was used to suggest cross-reactivity with CCL4 from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4 based on 90% homology. Cross-reactivity with CCL4 from other sources has not been determined. The swine CCL4 sequence is also 86% homologous to swine CCL3L1.
- Gene Name
- CCL4|CCL4L1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein raised in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1β protein.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution for 3 months. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl