LSBio
Polyclonal Rabbit anti-Human CCL4 / MIP-1 Beta Antibody (WB)

Anti-MIP-1beta Antibody - Western Blot. anti-Human MIP-1beta antibody shows detection of a band ~15 kD in size corresponding to recombinant human MIP-1beta. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

Polyclonal Rabbit anti-Human CCL4 / MIP-1 Beta Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Lyophilized
See all Human CCL4 / MIP-1 Beta antibodies →
$579.00each
Catalog Number: LS-C154095-100

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Overview

Description
MIP-1 Beta antibody LS-C154095 is an unconjugated rabbit polyclonal antibody to human MIP-1 Beta (CCL4). Validated for ELISA and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ACT2 | Act-2 | AT744.1 | C-C motif chemokine 4 | Chemokine (C-C motif) ligand 4 | CC chemokine ligand 4 | HC21 | LAG1 | MIP1B | MIP1B1 | LAG-1 | SCYA2 | Secreted protein G-26 | SIS-gamma | MIP-1-beta | MIP-1-beta(1-69) | Small-inducible cytokine A4 | T-cell activation protein 2 | CCL4 | G-26 | Lymphocyte-activation gene 1 | PAT 744 | Protein H400 | SCYA4
Recommended Dilution: ELISA
1:10000
Recommended Dilution: WB
1:1000
UniProt Number
P13236
SwissProt
P13236

Target

Target
Human CCL4 / MIP-1 Beta
Antigen Species
Human
Epitope
This antibody will recognize both native and recombinant human IL-9 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Gene Name
CCL4|CCL4L1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1β protein.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Concentration
1 mg/mL
Preservative
No
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.