Western blot analysis of extracts of various cell lines, using CASP8 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
CASP8 / Caspase 8 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C408191-20
Catalog Number: LS-C408191
For bulk orders or custom formulations:
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- Description
- Caspase 8 antibody LS-C408191 is an unconjugated rabbit polyclonal antibody to Caspase 8 (CASP8) from human. It is reactive with human and mouse. Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ALPS2B | Apoptotic protease Mch-5 | Apoptotic cysteine protease | Casp-8 | Caspase-8 | CAP4 | Caspase 8 | MACH | MCH5 | MORT1-associated ced-3 homolog | ICE-like apoptotic protease 5 | Pro Caspase 8 | CASP8 | FADD-like ICE | FLICE | MACH-alpha-1/2/3 protein | MACH-beta-1/2/3/4 protein
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q14790
- NCBI GenBank
- AAC50645.1 | nm_001228
- SwissProt
- Q14790
Target
- Target
- Human CASP8 / Caspase 8
- Antigen Species
- Human
- Epitope
- Human CASP8 / Caspase 8
- Gene Name
- CASP8
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-479 of human CASP8 (NP_001073593.1). MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEAD
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS