IHC analysis of Calpain 2 using anti-Calpain 2 antibody. Calpain 2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Calpain 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CAPN2 / Calpain 2 / M-Calpain Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C662194-100
Catalog Number: LS-C662194
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- Description
- M-Calpain antibody LS-C662194 is an unconjugated rabbit polyclonal antibody to human M-Calpain (CAPN2 / Calpain 2). Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Calpain 2, large subunit | Calpain ii | Calpain, large polypeptide L2 | Calpain-2 catalytic subunit | Calpain 2 | Calpain large polypeptide L2 | Calpain-2 large subunit | CANP 2 | CAPN2 | Calpain M-type | CANP2 | CANPL2 | CANPml | M-type calpain | M-calpain | MCANP | Millimolar-calpain
- UniProt Number
- P17655
- NCBI GenBank
- NM_001748 | NP_001739.2
- SwissProt
- P17655
Target
- Target
- Human CAPN2 / Calpain 2 / M-Calpain
- Antigen Species
- Human
- Epitope
- Ubiquitous.
- Gene Name
- CAPN2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5% and 92.5% amino acid (aa) sequence identity with mouse and rat Calpain 2, respectively.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- NaCl