Detection of Human BUB3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-BUB3 antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 minutes.

BUB3 Antibody (aa1-50)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$301.00each

Catalog Number: LS-C287258-20

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Overview

Description: BUB3 antibody LS-C287258 is an unconjugated rabbit polyclonal antibody to human BUB3 (aa1-50). Validated for IHC and WB.
Application: IHC, WB
Reactivity: Hu
Predicted Reactivity: Bovine, Mouse, Non-Human Primate
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Budding uninhibited by benomyl | BUB3 | HBUB3 | BUB3L | Mitotic checkpoint component
Recommended Dilution: IHC: 1:500 - 1:2000
Recommended Dilution: WB: 1:5000 - 1:15000
UniProt Number: O43684
NCBI GenBank: NM_004725 | NP_004716.1
SwissProt: O43684

Target

Target: Human BUB3
Antigen Species: Human
Epitope: Region between residues 1 and 50 of human Bub3 budding uninhibited by benzimidazoles 3 using the numbering given in Swiss-Prot entry O43684 (GeneID 9184).
Gene Name: BUB3

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: A portion of GeneID: 9184 corresponding to amino acids between 1 and 50 of UniprotID: O43684
Immunogen Type: Recombinant Protein Fragment