LSBio
BrdU Antibody

Immunofluorescence Microscopy of rabbit anti-BrdU antibody. Tissue: 293T cells transfected expressing GFP-MCM9 L. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Anti-rabbit ATTO550 secondary antibody at 1:10,000 for 45 min at RT. Localization: BrdU is nuclear. Staining: BrdU in merged image shows with green and purple fluorescent signal with DAPI nuclear counterstain.

BrdU Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Format:Liquid
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$311.00each
Catalog Number: LS-C745342-25

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Overview

Description
BrdU antibody LS-C745342 is an unconjugated rabbit polyclonal antibody to BrdU. Validated for ELISA, Flow, IF, IHC, IP and WB.
Application
ELISA, FC, IF, IHC, IP, WB
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: FC
1:50 - 1:100
Recommended Dilution: IF/ICC
1:500
Recommended Dilution: IHC
1:100 - 1:500

Target

Target
BrdU

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with Bromodeoxyuridine-KLH.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.