Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Immunofluorescence Microscopy of Mouse Anti-BrdU antibody. Tissue: OCT-embedded E10.5 mouse embryo. Localization: 40X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 1:500 in 0.4% PBS+Triton with 1% normal sheep serum overnight at 4 degrees C. Secondary antibody: Alexa Fluor 488 Anti-Mouse secondary antibody at 1:200 for 45 min at RT. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.
BrdU Antibody (clone 29G6.E8)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C209697-100
Catalog Number: LS-C209697
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- BrdU antibody LS-C209697 is an unconjugated mouse monoclonal antibody to BrdU. Validated for ELISA, Flow, IF, IHC and WB.
- Application
- ELISA, FC, IF, IHC, WB
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: FC
- 1:50 - 1:100
- Recommended Dilution: IF/ICC
- 1:500 - 1:3000
- Recommended Dilution: IHC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:2000 - 1:5000
Target
- Target
- BrdU
- Epitope
- This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 29G6.E8
- Isotype
- IgG1
- Light Chain
- Lambda Light Chain
Immunogen
- Immunogen
- Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with Bromodeoxyuridine-KLH followed by hybridoma development.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
