Formalin-fixed paraffin-embedded section of a human prostate cancer tissue array stained for Bcl-2 expression using Polyclonal Antibody to Bcl-2 at 1:2000. Hematoxylin-Eosin counterstain.
BCL2 / Bcl-2 Antibody (aa61-76)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C148231-50
Catalog Number: LS-C148231
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- Description
- Bcl-2 antibody LS-C148231 is an unconjugated rabbit polyclonal antibody to human Bcl-2 (BCL2) (aa61-76). Validated for IHC, IP and WB.
- Application
- IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Apoptosis regulator Bcl-2 | BCL2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
- Recommended Dilution: IHC-P
- 1:1000 - 1:5000
- Recommended Dilution: IP
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- P10415
- NCBI GenBank
- NM_000633 | NP_000624.2
- SwissProt
- P10415
Target
- Target
- Human BCL2 / Bcl-2
- Antigen Species
- Human
- Epitope
- Bcl-2.
- Gene Name
- BCL2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic peptide corresponding to amino acids 61-76 (ASRDPVARTSPLQTPAC) of human Bcl-2. This sequence is 100% conserved in human Bcl-2 alpha (239 amino acids) and beta (205 amino acids) isoforms. The immunogen sequence is not conserved between human Bcl-2 and most other species. Therefore this antibody may be particularly useful for detecting human Bcl-2 in transgenic studies. Percent identity with other species by BLAST analysis: Human (100%), Gorilla (94%), Gibbon (94%), Monkey (94%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.05% sodium azide
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.