
Western blot analysis for Puma a(CT) using antibody at 1:500 against 30 ug/lane of A)Jurkat and B) NIH 3T3whole cell lysate.
BBC3 / PUMA Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C779-100
Catalog Number: LS-C779
For bulk orders or custom formulations:
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- Description
- PUMA antibody LS-C779 is an unconjugated rabbit polyclonal antibody to human PUMA (BBC3). Validated for IHC and WB.
- Application
- IHC, IHC-P, WB
- Reactivity
- Hu
- Predicted Reactivity
- Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BBC3 | Bcl-2-binding component 3 | BCL2 binding component 3 | JFY-1 | JFY1 | PUMA | PUMA alpha | PUMA a
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q9BXH1
- NCBI GenBank
- NM_014417 | NP_055232.1
- SwissProt
- Q9BXH1
Target
- Target
- Human BBC3 / PUMA
- Antigen Species
- Human
- Epitope
- The amino acid sequence used as immunogen is 100% homologous in human (PUMA a and b) and 78% homologous in mouse and rat. Human PUMA a is a 193 amino acid protein with a predicted molecular weight of approximately 22 kD. Human PUMA b is a 131 amino acid protein with a predicted molecular weight of approximately 15 kD.
- Gene Name
- BBC3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A portion of amino acids 186-199 (PLPRGHRAPEMEPN) of human PUMA a. Percent identity by BLAST analysis: Human, Monkey (100%); Marmoset (93%); Ferret, Panda, Dog, Horse, Pig (86%); Mouse, Rat (79%).
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.05% Sodium Azide, 0.2% Gelatin
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: 4°C; Long term: -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
