Anti-BBC3 / PUMA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

IHC-plus™ BBC3 / PUMA Antibody (aa2-16)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$505.00each

Catalog Number: LS-B7285-0.05

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Overview

Description: PUMA antibody LS-B7285 is an unconjugated rabbit polyclonal antibody to human PUMA (BBC3) (aa2-16). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: IHC, IHC-P, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: BBC3 | Bcl-2-binding component 3 | BCL2 binding component 3 | JFY-1 | JFY1 | PUMA | PUMA alpha
Recommended Dilution: IHC-P: 2.5 µg/ml
Recommended Dilution: WB: 2 - 4 µg/ml
UniProt Number: Q9BXH1
NCBI GenBank: NM_014417 | NP_055232.1
SwissProt: Q9BXH1

Target

Target: BBC3 / PUMA
Antigen Species: Human
Specificity: Recognizes the p53 - upregulated modulator of apoptosis (PUMA), also known as bbc3. PUMA is a pro-apoptotic member of the BCL-2 family. PUMA is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53
Epitope: Recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kD pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. Four isoforms of PUMA have been described due to alternative splicing. Is expected to recognize the canonical isofom 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.
Gene Name: BBC3

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-Terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%); Opossum (87%).
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.02% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Other

Storage & Handling

Storage Conditions: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer: PBS