Western blot of anti-Puma BH3 domain antibody in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
BBC3 / PUMA Antibody (aa119-154)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C98860-400
Catalog Number: LS-C98860
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- Description
- PUMA antibody LS-C98860 is an unconjugated rabbit polyclonal antibody to PUMA (BBC3) (aa119-154) from human. It is reactive with human and mouse. Validated for Flow, IF, IHC and WB. Cited in 1 publication.
- Application
- FC, IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BBC3 | Bcl-2-binding component 3 | BCL2 binding component 3 | JFY-1 | JFY1 | PUMA | PUMA alpha
- Recommended Dilution: FC
- 1:10 - 1:50
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- Recommended Dilution: IHC-P
- 1:10 - 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9BXH1
- NCBI GenBank
- NM_014417 | NP_055232.1
- SwissProt
- Q9BXH1
Target
- Target
- Human BBC3 / PUMA
- Antigen Species
- Human
- Epitope
- This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.
- Gene Name
- BBC3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Ammonium Sulfate Precipitation
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
Bcl-2 Is a Therapeutic Target for Hypodiploid B-Lineage Acute Lymphoblastic Leukemia. Ernesto Diaz-Flores, Evan Q Comeaux, Kailyn L Kim, Ella Melnik, Kyle Beckman, Kara L Davis, Kevin Wu, Jon Akutagawa, Olga Bridges, Roberta Marino, Margo Wohlfeil, Benjamin S Braun, Charles G Mullighan, Mignon L Loh. Cancer research. 2019 May;79:2339-2351. PubMed: 30862722 PMC: PMC6497548