Immunofluorescence staining of ACTL6A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ACTL6A polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
BAF53 / ACTL6A Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C814695-100
Catalog Number: LS-C814695
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- Description
- ACTL6A antibody LS-C814695 is an unconjugated rabbit polyclonal antibody to human ACTL6A (BAF53). Validated for ICC, IF and WB.
- Application
- ICC, IF, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ACTL6 | Actin-like 6A | Actin-related protein 4 | Actin-related protein Baf53a | ArpNbeta | BAF53 | ARPN-BETA | BAF53A | BRG1-associated factor 53A | Actin-like protein 6A | ACTL6A | INO80 complex subunit K | ARP4 | BAF complex 53 kDa subunit | HArpN beta | INO80K
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- O96019
- NCBI GenBank
- NM_004301 | NP_004292.1
- SwissProt
- O96019
Target
- Target
- Human BAF53 / ACTL6A
- Antigen Species
- Human
- Epitope
- Human Actin-like protein 6A/BAF53a
- Gene Name
- ACTL6A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the center region of the Human Actin-like protein 6A/ACTL6A/BAF53a
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS