Anti-ATR Antibody - Western Blot. Western blot of anti-ATR antibody shows detection of ATR in HeLa cell nuclear extract (lane 1). Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 ug peptide for 1 h at room temperature followed by centrifugation). A 4-8% gradient gel was used for separation. Goat serum was used at 5% for blocking. The arrowhead corresponds to 301 kD ATR when compared to MW markers (Lane M). The primary antibody was used at a 1:1400 dilution.

ATR Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$579.00each

Catalog Number: LS-C60116-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: ATR antibody LS-C60116 is an unconjugated rabbit polyclonal antibody to ATR from human. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
Application: ELISA, IHC, WB
Reactivity: Hu
Predicted Reactivity: Dog, Fish (Other), Mouse, Non-Human Primate, Rat, Xenopus
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: ATR | FRAP-related protein-1 | FCTCS | SCKL1 | MEC1 | Protein kinase ATR | Rad3 related protein | SCKL | FRAP-related protein 1 | FRP1 | XATR | Serine/threonine-protein kinase ATR | Ataxia telangiectasia and Rad3-related protein | mitosis entry checkpoint 1
Recommended Dilution: ELISA: 1:15000 - 1:70000
Recommended Dilution: WB: 1:1000 - 1:5000
UniProt Number: Q13535
NCBI GenBank: NM_001184 | NP_001175.2
SwissProt: Q13535

Target

Target: Human ATR
Antigen Species: Human
Epitope: This antibody is specific for human ATR protein. A BLAST analysis was used to suggest cross-reactivity with ATR from human, mouse, rat, monkey, dog, fish and Xenopus sources based on a 100% homology with the immunizing sequence. Cross-reactivity with ATR from other sources has not been determined.
Gene Name: ATR

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1.28 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl