Detection of Human ARID1A by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-ARID1A antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
ARID1A / BAF250 Antibody (aa675-725)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287870-2
Catalog Number: LS-C287870
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- Description
- BAF250 antibody LS-C287870 is an unconjugated rabbit polyclonal antibody to human BAF250 (ARID1A) (aa675-725). Validated for WB. Cited in 1 publication.
- Application
- IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ARID1A | BAF250 | BAF250a | BRG1-associated factor 250a | C1orf4 | BRG1-associated factor 250 | ELD | HOSA1 | Osa homolog 1 | OSA1 | OSA1 nuclear protein | SMARCF1 | MRD14 | p270 | B120 | BM029 | Brain protein 120 | C10rf4 | HELD | SWI-like protein | SWI/SNF complex protein p270
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- O14497
- NCBI GenBank
- NM_006015 | NP_006006.3
- SwissProt
- O14497
Target
- Target
- Human ARID1A / BAF250
- Antigen Species
- Human
- Epitope
- Region between residue 675 and 725 of human AT rich interactive domain 1A (BRG1-associated factor 250kD) using the numbering given in entry NP_006006.3 (GeneID 8289).
- Gene Name
- ARID1A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 8289 corresponding to amino acids between 675 and 725 of UniprotID: O14497
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- TBS, 0.09% Sodium Azide, 0.1% BSA
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- TBS
Arid1a Has Context-Dependent Oncogenic and Tumor Suppressor Functions in Liver Cancer. Sun X, Wang SC, Wei Y, Luo X, Jia Y, Li L, Gopal P, Zhu M, Nassour I, Chuang JC, Maples T, Celen C, Nguyen LH, Wu L, Fu S, Li W, Hui L, Tian F, Ji Y, Zhang S, Sorouri M, Hwang TH, Letzig L, James L, Wang Z, Yopp AC, Singal AG, Zhu H. Cancer cell. 2017 November;32:574-589. PubMed: 29136504 PMC: PMC5728182