Western Blot of rabbit anti Alkaline Phosphatase antibody Lane 1: Calf intestine Alkaline Phosphatase. Load: ~1.5 ug Block: 5% Blotto 1 hour at 4 degrees C. Primary antibody: diluted to 1:1000 in 5% Blotto overnight at 4 degrees C. Secondary antibody: Goat anti Rabbit LS-C60865 Lot#21231 1:20,000 in MB-070 1 Hour 4 degrees C Substrate: Femtomax 110.
ALPI / Alkaline Phosphatase Antibody (Lyophilized)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine |
| Format: | Lyophilized |
$579.00each
Catalog Number: LS-C209223-100
Catalog Number: LS-C209223
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- Description
- Alkaline Phosphatase antibody LS-C209223 is an unconjugated rabbit polyclonal antibody to bovine Alkaline Phosphatase (ALPI). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Bov
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Alkaline phosphomonoesterase | ALPI | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
- Recommended Dilution: ELISA
- 1:20000 - 1:100000
- Recommended Dilution: IHC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P09923
- NCBI GenBank
- NM_001631 | NP_001622.2
- SwissProt
- P09923
Target
- Target
- Bovine ALPI / Alkaline Phosphatase
- Antigen Species
- Bovine
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.
- Gene Name
- ALPI
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Alkaline Phosphatase [Calf intestine].
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl