Western Blot of rabbit anti-Alkaline Phosphatase antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (calf intestine). Load: 50 ng per lane. Primary antibody: Alkaline Phosphatase antibody Biotin conjugated at 1:2,000 for 60 min at RT. Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: 57 kDa, 57 kDa for Alkaline Phosphatase.
ALPI / Alkaline Phosphatase Antibody (Liquid, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Bovine |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745122-25
Catalog Number: LS-C745122
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Alkaline Phosphatase antibody LS-C745122 is a biotin-conjugated rabbit polyclonal antibody to bovine Alkaline Phosphatase (ALPI). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Bov
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Alkaline phosphomonoesterase | ALPI | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
- Recommended Dilution: ELISA
- 1:1000 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P09923
- NCBI GenBank
- NM_001631, NP_001622.2
- SwissProt
- P09923
Target
- Target
- Bovine ALPI / Alkaline Phosphatase
- Antigen Species
- Bov
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]. Cross reactivities against Alkaline Phosphatase from other sources may occur but have not been specifically determined.
- Gene Name
- ALPI
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Alkaline Phosphatase [Calf intestine].
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- IEX
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl