Western Blot of Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody Peroxidase Conjugated. Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a.
ALPI / Alkaline Phosphatase Antibody (Lyophilized, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | E. coli |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C209645-100
Catalog Number: LS-C209645
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- Description
- Alkaline Phosphatase antibody LS-C209645 is an HRP-conjugated rabbit polyclonal antibody to e. coli Alkaline Phosphatase (ALPI). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- E.col
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Alkaline phosphomonoesterase | ALPI | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
- Recommended Dilution: ELISA
- 1:4000 - 1:20000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P09923
- NCBI GenBank
- NM_001631 | NP_001622.2
- SwissProt
- P09923
Target
- Target
- E. coli ALPI / Alkaline Phosphatase
- Antigen Species
- E. coli
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against mammalian forms of Alkaline Phosphatase.
- Gene Name
- ALPI
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Alkaline Phosphatase [E.coli]
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl