Dot Blot for Anti-ALKALINE PHOSPHATASE (Human Intestine) (RABBIT) Antibody Biotin Conjugated. Lane 1: 100 ng Alkaline Phosphatase. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: Anti-Alkaline Phosphatase Antibody Biotin Conjugated at 10 µg/ml at RT for 1 hour. Secondary Antibody: Streptavidin Peroxidase Conjugated at a 1:40,000 dilution at RT for 30 minutes. Block: MB-070 at RT for 30 minutes.
ALPI / Alkaline Phosphatase Antibody (Liquid, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745059-25
Catalog Number: LS-C745059
For bulk orders or custom formulations:
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- Description
- Alkaline Phosphatase antibody LS-C745059 is a biotin-conjugated rabbit polyclonal antibody to human Alkaline Phosphatase (ALPI). Validated for DB, ELISA and WB.
- Application
- DB, ELISA, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Alkaline phosphomonoesterase | ALPI | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
- UniProt Number
- P09923
- NCBI GenBank
- NM_001631, NP_001622.2
- SwissProt
- P09923
Target
- Target
- Human ALPI / Alkaline Phosphatase
- Antigen Species
- Hu
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Human Intestine]. Cross reactivity against Alkaline Phosphatase from other sources may occur but has not been specifically determined.
- Gene Name
- ALPI
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Alkaline Phosphatase [human intestine].
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- IEX
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl