Western Blot of rabbit Anti-Alkaline Phosphatase Primary Antibody. Lane 1: Alkaline Phosphatase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alkaline Phosphatase primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 58 kDa, 58 kDa for Alkaline Phosphatase. Other band(s): None.
ALPI / Alkaline Phosphatase Antibody (Lyophilized)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$579.00each
Catalog Number: LS-C744967-100
Catalog Number: LS-C744967
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Alkaline Phosphatase antibody LS-C744967 is an unconjugated rabbit polyclonal antibody to human Alkaline Phosphatase (ALPI). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Alkaline phosphomonoesterase | ALPI | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
- Recommended Dilution: ELISA
- 1:4000 - 1:20000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P09923
- NCBI GenBank
- NM_001631 | NP_001622.2
- SwissProt
- P09923
Target
- Target
- Human ALPI / Alkaline Phosphatase
- Antigen Species
- Human
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Human Intestine]. Cross reactivity against Alkaline Phosphatase from other sources is unknown.
- Gene Name
- ALPI
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Alkaline Phosphatase [human intestine].
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl