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AKT2 Antibody (Ser474)

Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (S474). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AKT2 immunoreactivity is localized to Cytoplasm significantly.

AKT2 Antibody (Ser474)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human AKT2 antibodies →
$401.00each
Catalog Number: LS-C98942-400

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Overview

Description
AKT2 antibody LS-C98942 is an unconjugated rabbit polyclonal antibody to human AKT2 (Ser474). Validated for IF, IHC and WB.
Application
IF, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Mouse, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AKT2 | PKB beta | PKBBETA | PRKBB | Protein kinase Akt-2 | Protein kinase B beta | RAC-PK-beta | Rac protein kinase beta | RAC-BETA | HIHGHH | PKBB
Recommended Dilution: IF/ICC
1:10 - 1:50
Recommended Dilution: IHC-P
1:10 - 1:50
Recommended Dilution: WB
1:1000
UniProt Number
P31751
SwissProt
P31751

Target

Target
Human AKT2
Antigen Species
Human
Epitope
This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-481 amino acids from human AKT2.
Gene Name
AKT2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Volume
400 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.