Western Blot of Rat anti-AKT2 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT2 antibody at 1:1,000 for overnight at 4 degrees C. Secondary antibody: Peroxidase rat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none. This image was taken for the unmodified form of this product. Other forms have not been tested.

AKT2 Antibody (Internal, clone 16G11.E8)

Primary AntibodyLSBio Guarantee

Antibody:	Rat Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$2652.00each

Catalog Number: LS-C209265-1

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: AKT2 antibody LS-C209265 is an unconjugated rat monoclonal antibody to human AKT2 (Internal). Validated for ELISA, Flow, IHC and WB.
Application: ELISA, FC, IHC, WB
Reactivity: Hu
Predicted Reactivity: Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: AKT2 | PKB beta | PKBBETA | PRKBB | Protein kinase Akt-2 | Protein kinase B beta | RAC-PK-beta | Rac protein kinase beta | RAC-BETA | HIHGHH | PKBB
Recommended Dilution: ELISA: 1:2000 - 1:10000
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P31751
NCBI GenBank: NM_001626 | NP_001617.1
SwissProt: P31751

Target

Target: Human AKT2
Antigen Species: Human
Epitope: The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
Gene Name: AKT2

Antibody

Host Species: Rat
Clonality: Monoclonal
Clone: 16G11.E8
Isotype: IgG2a
Light Chain: Kappa Light Chain

Immunogen

Immunogen: Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Concentration: 1 mg/mL
Preservative: No
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl