Western blot analysis of extracts of HeLa cells, using Phospho-AKT1S1-T246 antibody at 1:2000 dilution. HeLa cells were treated by Insulin (0.01U/ml) for 15 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 90s.

AKT1S1 / PRAS40 Antibody (phospho-Thr246)

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Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

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$293.00each

Catalog Number: LS-C750601-20

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Overview

Description: PRAS40 antibody LS-C750601 is an unconjugated rabbit polyclonal antibody to PRAS40 (AKT1S1) (pThr246) from human. It is reactive with human and mouse. Validated for WB.
Application: WB
Reactivity: Hu, Ms
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: AKT1S1 | PRAS40 | Proline-rich AKT1 substrate 1 | Lobe
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q96B36
NCBI GenBank: NM_032375, NP_115751.3
SwissProt: Q96B36