Western blot analysis of extracts of HeLa cells, using Phospho-AKT1S1-T246 antibody at 1:2000 dilution. HeLa cells were treated by Insulin (0.01U/ml) for 15 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 90s.
AKT1S1 / PRAS40 Antibody (phospho-Thr246)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$293.00each
Catalog Number: LS-C750601-20
Catalog Number: LS-C750601
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- PRAS40 antibody LS-C750601 is an unconjugated rabbit polyclonal antibody to PRAS40 (AKT1S1) (pThr246) from human. It is reactive with human and mouse. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AKT1S1 | PRAS40 | Proline-rich AKT1 substrate 1 | Lobe
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q96B36
- NCBI GenBank
- NM_032375 | NP_115751.3
- SwissProt
- Q96B36
Target
- Target
- Human AKT1S1 / PRAS40
- Antigen Species
- Human
- Gene Name
- AKT1S1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS