Western blot analysis of extracts from HepG2 cells, treated with PDGF (50ng/ml, 30mins), using Akt1 S1 (Phospho-Thr246) antibody.
AKT1S1 / PRAS40 Antibody (phospho-Thr246)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C821803-100
Catalog Number: LS-C821803
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- Description
- PRAS40 antibody LS-C821803 is an unconjugated rabbit polyclonal antibody to PRAS40 (AKT1S1) (pThr246) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AKT1S1 | PRAS40 | Proline-rich AKT1 substrate 1 | Lobe
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q96B36
- NCBI GenBank
- NM_032375 | NP_115751.3
- SwissProt
- Q96B36
Target
- Target
- Human AKT1S1 / PRAS40
- Antigen Species
- Human
- Epitope
- Akt1 S1 (Phospho-Thr246) antibody detects endogenous levels of AKT1S1 only when phosphorylated at threonine 246. (Human: Thr246; Mouse: Thr247; Rat: Thr262)
- Gene Name
- AKT1S1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human AKT1S1 around the phosphorylation site of threonine 246 (L-N-TP-S-D).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl