Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 un-active recombinant protein. Load: 25 ng per lane. Primary antibody: Akt phospho T308 Biotin Conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho T308. Other band(s): none This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKT1 Antibody (phospho-Thr308, clone 18F3.H11, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Non-Human Primate, Rat |
| Format: | Lyophilized |
$482.00each
Catalog Number: LS-C153790-50
Catalog Number: LS-C153790
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- AKT1 antibody LS-C153790 is a biotin-conjugated mouse monoclonal antibody to AKT1 (pThr308) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC, IP and WB.
- Application
- ELISA, FC, IHC, IP, WB
- Reactivity
- Hu, Ms, NHP, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- P31749
- NCBI GenBank
- NM_005163 | NP_005154.2
- SwissProt
- P31749
Target
- Target
- Human AKT1
- Antigen Species
- Human
- Epitope
- This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
- Gene Name
- AKT1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 18F3.H11
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
