Western Blot - Anti-AKT Antibody. Anti-AKT Antibody in western blot showing detection of endogenous protein in whole cell extracts from H2O2 treated MCF7 cells (lane 2). Recombinant AKT is detected in lane 3. Lanes 1 and 4 contain wcl from HeLa and A431 cells, respectively. Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown). The band at ~55-60 kD, indicated by the arrowhead, corresponds to the expected molecular weight of AKT. AKT antibody was diluted 1:300 in 1% BLOTTO and reacted with the membrane overnight at 4° C. IRDYE800 conjugated Dnky-a-Sheep IgG was used at a 1:10000 dilution for 45 min at room temperature.

AKT1 Antibody (Internal)

Primary AntibodyLSBio Guarantee

Antibody:	Sheep Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$579.00each

Catalog Number: LS-C60161-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: AKT1 antibody LS-C60161 is an unconjugated sheep polyclonal antibody to AKT1 (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
Application: ELISA, FC, IF, IHC, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Recommended Dilution: ELISA: 1:5000 - 1:20000
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P31749
NCBI GenBank: NM_005163 | NP_005154.2
SwissProt: P31749

Target

Target: Human AKT1
Antigen Species: Human
Epitope: This antibody is directed against AKT and reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse and chicken AKT.
Gene Name: AKT1

Antibody

Host Species: Sheep
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Anti-AKT antibody was affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein. A residue of cysteine was added to facilitate coupling.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Concentration: 0.9 mg/mL
Preservative: Yes
Purification Method: Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions: Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl