Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 78 kDa for AKT1. Other band(s): none. This image was taken for the unmodified form of this product. Other forms have not been tested.

AKT1 Antibody (Internal, clone 5E5.F5.D7)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$586.00each

Catalog Number: LS-C209208-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: AKT1 antibody LS-C209208 is an unconjugated mouse monoclonal antibody to human AKT1 (Internal). Validated for ELISA, Flow, IHC and WB.
Application: ELISA, FC, IHC, WB
Reactivity: Hu
Predicted Reactivity: Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Recommended Dilution: ELISA: 1:2000 - 1:10000
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P31749
NCBI GenBank: NM_005163 | NP_005154.2
SwissProt: P31749

Target

Target: Human AKT1
Antigen Species: Human
Epitope: The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
Gene Name: AKT1

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 5E5.F5.D7
Isotype: IgG2b
Light Chain: Kappa Light Chain

Immunogen

Immunogen: Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl