Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Plate was coated with monoclonal anti AKT1 antibody followed by incubation with recombinant AKT1, AKT2, AKT3 proteins. Binding was detected with biotinylated monoclonal anti AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3
AKT1 Antibody (Internal, clone 14E5.A2.B2.H9, APC)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Lyophilized |
$594.00each
Catalog Number: LS-C744943-50
Catalog Number: LS-C744943
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- AKT1 antibody LS-C744943 is an APC-conjugated mouse monoclonal antibody to AKT1 (Internal) from human. It is reactive with human, mouse and rat. Validated for DB, ELISA, Flow, IF, IHC and WB.
- Application
- DB, ELISA, FC, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
- Recommended Dilution: FC
- 1 ug/ml
- UniProt Number
- P31749
- NCBI GenBank
- NM_005163 | NP_005154.2
- SwissProt
- P31749
Target
- Target
- Human AKT1
- Antigen Species
- Human
- Epitope
- The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
- Gene Name
- AKT1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 14E5.A2.B2.H9
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Allophycocyanin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.5 M NaCl
