Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.

AKT1 Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$2652.00each

Catalog Number: LS-C744832-1

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: AKT1 antibody LS-C744832 is an unconjugated mouse monoclonal antibody to AKT1 (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB.
Application: ELISA, FC, IHC, WB
Reactivity: Hu
Predicted Reactivity: Mouse, Non-Human Primate, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Recommended Dilution: ELISA: 1:20000
Recommended Dilution: IHC: 20 µg/ml
Recommended Dilution: WB: 1:500 - 1:3000
UniProt Number: P31749
NCBI GenBank: NM_005163 | NP_005154.2
SwissProt: P31749

Target

Target: Human AKT1
Antigen Species: Human
Epitope: This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
Gene Name: AKT1

Antibody

Host Species: Mouse
Clonality: Monoclonal
Isotype: IgG1
Light Chain: Kappa Light Chain
Origin Species: Human

Immunogen

Immunogen: Anti-Akt monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human AKT1 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl